Oocyte and sperm retrieval

Oocyte Retrieval
The oocyte retrieval (egg retrieval) is performed under general anesthesia 34-36 hours after the application of hCG (Pregnyl).

The retrieval is performed by passing a hollow needle through the wall of vagina to the ovary. The needle is guided into the ovary with the use of vaginal ultrasound probe. The fluid is aspirated from the follicle and deposited into a test tube. The number and quality of eggs is immediately assessed in the embryology lab.

Sperm Retrieval

The sperm retrieval is performed on the very same day as the oocyte retrieval.
The sperm retrieval is carried out through masturbation in a quiet separate room. If necessary, the partner can be present. The retrieval can be also performed outside of our facility – it is necessary to withdraw a sterile container and information about a correct manipulation with the collected semen from the andrology laboratory in advance.

Surgical sperm retrieval can also be carried out – fluid from the epididymal tube containing immature sperm is drained out.

MESA technique is used (Micro-Epididymal Sperm Aspiration). This procedure is performed in case the sperm transport between the epididymis and the urethra fails.
If sperm is created, it can be collected from the epithelium duct. The procedure is carried out when sperm is not released or is not transported from the epididymis to the urethra.

– TESE (Testicular Sperm Extraction) – it is the microsurgical removal of testicular tissue from which sperm is extracted. The sperm from frozen samples can be also used for egg fertilization. For example, it is possible to freeze the sperm before chemotherapy or after a TESE procedure and defrost it on the day of egg retrieval and use it for fertilization.

The retrieved eggs can also be fertilized by an anonymous donor´s sperm – see the chapter  about sperm donation for more details.